2XReadyGoHSFPCRMasterMix isa2Xconcentratedsolutionofhot-startandhighfidelity Taq DNAPolymerases,dNTPs,andallofthecomponentsrequiredforPCR,exceptDNAtemplateandprimers.Thispre-mixedformulationsavestimeandreducescontaminationduetoareducednumberofpipettingstepsrequiredforPCRsetup.Itincreasesspecificityandsensitivitybecausereducingformationofnospecificproductsandprimerdimer.Youcansetupreactionsatroomtemperature.ThemixisoptimizedforefficientandreproducIBLePCR.
TheMasterMixhasfourversions,withstandardbufferandGCbuffer,colorlessandGreen.TheMasterMixwithGCbufferisusedforamplificationofGC-richtarget(GCcontentis>65%).ThegreenoneissupplementedwithtwotrackingdyesfortheconvenienceofdirectgelloADIngofPCRproducts.
Highlights
- Convenient: Ready-to-usemix,directloadingofPCRproductsongel
- Thermostable: Retainatleast85%activityafter60minat95°C
- GeneratesPCRproductswith3"-dAoverhangs
- Incorporatesmodifiednucleotides (e.g.,biotin-,digoxigenin-,fluorescently-labelednucleotide)
DataSheet: 2XReadyGoHSFPCRMasterMix