OmniKlenaq isamutantofKlenTaqDNApolymerasethatmakestheenzymeresistanttotheinhibitoryeffectsofblood,soilandmore.Ittypicallyremainsfunctionalin20-25%wholeblood,especiallyinthepresenceofoneofourPCRenhancercocktails,andinsomeconcentrationsofcrudesoilextractswhereothercommercialenzymesfail. Thisenzymecanbeusedinreal-timePCRwithDNAbindingdyes,suchasSYBRGreenandEvaGreen,however,itcannotbeusedinreal-timePCRTaqManassaywhichrequires5’-3’exonucleaseactivity.
OmniKlentaqLA version(Long&Accurate)isavailable, whichallowsforamplificationoflongerproductswithhigherfidelityandaccuracy.
OmniKlentaqandOmniKlentaqLAMix areavailableasaready-to-use2XmixtureofDNApolymerase,salts,magnesiumanddNTPsforsettingupatrouble-freePCRreactionandthereisthechoiceofaninertgreendye,usedfordirectgelloADIng.Allyouhavetodoistoaddtemplate,specificprimersandwater(Clickhere).
- ThermostABIlity –morethermostableandimprovedfidelitythanTaqDNApolymerase
- Efficient–novelbuffersystemmaximizesefficiencyofPCRamplification,increasesyieldofanyPCRproduct
- RobustandInhibition-resistant–reliableamplificationinthepresenceofinhibitorsandwitheventhemostchallengingDNAtargets,especiallywhencombiningwithourpowerfulPCRenhancers
- FlexIBLe–LAversionisidealforamplifyinganytargetupto30kbfromDNAextractedfromhuman,animalandplantsamples
DataSheet:OmniKlenTaq,OmniKlenTaqLA