SoloTaqTMisanewmutantofKlentaqpolymerasethatmakestheenzymeresistanttotheinhibitoryeffectsofhigherconcentrationsofblood,soil,plantandmore.Ittypicallyremainsfunctionalin40%wholebloodinPCR,andinsomeconcentrationsofcrudesoilextractswhereothercommercialenzymesfail.Itisabletoamplifythetargetgenedirectlyfromwholeblood,serum,plasma,water,milk,tissueandplantleaf,etc.withoutDNApurificationpriortoPCR.Thisenzymecanbeusedinreal-timePCRwithDNAbindingdyes,suchasSYBRGreenandEvaGreen,however,itcannotbeusedinreal-timePCRTaqManassaywhichrequires5’-3’exonucleaseactivity.
SoloTaqTM LA version (Long&Accurate)isavailable,whichallowsforamplificationoflongerproductswithhigherfidelityandaccuracy.
SoloTaqTM&SoloTaqTM LAMix are availableasaready-to-use2XmixtureofDNApolymerase,salts,magnesiumanddNTPsforsettingupatrouble-freePCRreactionandthereisthechoiceofaninertgreendye,usedfordirectgelloADIng.Allyouhavetodoistoaddtemplate,specificprimersandwater(Clickhere).
- ThermostABIlity–morethermostablethanstandardTaqDNApolymerase
- Efficient–novelbuffersystemmaximizesefficiencyofPCRamplification,increasesyieldofanyPCRproduct
- RobustandInhibition-resistant–reliabledirectamplificationfromcrudesamplesandwitheventhemostchallengingDNAtargets,especiallywhencombiningwithourPCRenhancers
- FlexIBLe–LAversionisidealforamplifyinganytargetupto30kbfromDNAextractedfromhuman,animalandplantsamples
DataSheet:SoloTaq,SoloTaqLA
