AlphaTaqTMisanewgenerationmutantofTaqpolymerase.ThemutationsinTaqpolymerasegenerendertheenzymeresistanttotheinhibitoryeffectsofhigherconcentrationsofblood,soil,plantandmore.Ittypicallyremainsfunctionalin20-30%wholebloodinPCR,andinsomeconcentrationsofcrudesoilextractswhereothercommercialenzymesfail.ItcantoleratemoreinthepresenceofPCRenhancercocktail.Itisabletoamplifythetargetgenedirectlyfromwholeblood,serum,plasma,water,milk,tissueandplantleaf,etc.withoutDNApurificationpriortoPCR.Thisenzymecanbeusedinreal-timePCRwithbothDNAbindingdyes,suchasSYBRGreenandEvaGreen,andTaqManassaywhichrequires5’-3’exonucleaseactivity.
AlphaTaqTMLA version(Long&Accurate)isavailable,whichallowsforamplificationoflongerproductswithhigherfidelityandaccuracy.
AlphaTaqTMandAlphaTaqTM LAMix are availableasaready-to-use2XmixtureofDNApolymerase,salts,magnesiumanddNTPsforsettingupatrouble-freePCRreactionandthereisthechoiceofaninertgreendye,usedfordirectgelloADIng.Allyouhavetodoistoaddtemplate,specificprimersandwater(Clickhere).
- ThermostABIlity – morethermostablethanstandardTaqDNApolymerase
- Efficient–novelbuffersystemmaximizesefficiencyofPCRamplification,increasesyieldofanyPCRproduct
- RobustandInhibition-resistant–reliabledirectamplification fromcrudesamplesandwitheventhemostchallengingDNAtargets,especiallywhencombiningwithourpowerfulPCRenhancers
- FlexIBLe–LAversionisidealforamplifyinganytargetupto30kbfromDNAextractedfromhuman,animalandplantsamples