SupremTaqTMisanewgenerationmutantofTaqpolymerase.ThemutationsinTaqpolymerasegenerendertheenzymeresistanttotheinhibitoryeffectsofhigherconcentrationsofblood,soil,plantandmore.Ittypicallyremainsfunctionalin40%wholebloodinPCR,andinsomeconcentrationsofcrudesoilextractswhereothercommercialenzymesfail.ItcantoleratemoreinthepresenceofPCRenhancercocktail.Itisabletoamplifythetargetgenedirectlyfromwholeblood,serum,plasma,water,milk,tissueandplantleaf,etc.withoutDNApurificationpriortoPCR.Thisenzymecanbeusedinreal-timePCRwithbothDNAbindingdyes,suchasSYBRGreenandEvaGreen,andTaqManassaywhichrequires5’-3’exonucleaseactivity.
SupremTaqTMLA version(Long&Accurate)isavailable,which allowsforamplificationoflongerproductswithhigherfidelityandaccuracy.
SupremTaqTMandSupremTaqTM LAMix are availableasaready-to-use2XmixtureofDNApolymerase,salts,magnesiumanddNTPsforsettingupatrouble-freePCRreactionandthereisthechoiceofaninertgreendye,usedfordirectgelloADIng.Allyouhavetodoistoaddtemplate,specificprimersandwater(Clickhere).
- ThermostABIlity–morethermostablethanstandardTaqDNApolymerase
- Efficient–novelbuffersystemmaximizesefficiencyofPCRamplification,increasesyieldofanyPCRproduct
- RobustandInhibition-resistant–reliabledirectamplification fromcrudesampleswithoutDNApurificationandwitheventhemostchallengingDNAtargets,especiallywhencombiningwithourpowerfulPCRenhancers
- FlexIBLe–LAversionisidealforamplifyinganytargetupto30kbfromDNAextractedfromhuman,animalandplantsamples
DataSheet:SupremTaq,SupremTaqLA
